Fig. 4

a, Axial maximum projection showing the whole zebrafish larva tail at 24 hpf, nuclei labeled with tg(h2afva:h2afva-mCherry). Imaging volume is 1,064 × 532 × 287 μm consisting of 4,000 × 2,000 × 360 voxels per view for a total of 5.7 billion voxels acquired every 40 s. Scale bar, 100 μm. b, Side projection illustrating how the two light-sheets enter the sample at 45° to reach a given point in the sample. Depending on the sample geometry and placement, one of the two light-sheets will have a shorter path to reach that point and hence be less susceptible to absorption, refraction or scattering. Consequently, the corresponding view’s image will be more complete and better contrasted. c, Example regions (single xy plane slices) that demonstrate the complementarity of the two views. In some regions (left) the first view has better image quality, whereas in other regions (right) the second view is better. Scale bar, 3 μm. d, After registration, the two views can be fused together to obtain one high-quality image. e, Time-lapse max-projection frames over a 2.2 h period centered on the dorsomedial tail, during which time the boundary between neighboring somites are accentuated. Scale bar, 80 μm. f, Spatio-temporal zoom centered around a cell division, single xy plane slice. Despite the large field of view, both views are acquired every 40 s making it possible to follow the intermediate steps during mitosis—an important capability for achieving, for example, accurate lineage tracking. Scale bar, 10 μm.