PUBLICATION
DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
- Authors
- Yang, B., Lange, M., Millett-Sikking, A., Zhao, X., Bragantini, J., VijayKumar, S., Kamb, M., Gómez-Sjöberg, R., Solak, A.C., Wang, W., Kobayashi, H., McCarroll, M.N., Whitehead, L.W., Fiolka, R.P., Kornberg, T.B., York, A.G., Royer, L.A.
- ID
- ZDB-PUB-220323-31
- Date
- 2022
- Source
- Nature Methods 19(4): 461-469 (Journal)
- Registered Authors
- Royer, Loic
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain*/diagnostic imaging
- Drosophila
- Embryonic Development
- Microscopy, Fluorescence/methods
- Zebrafish*
- PubMed
- 35314838 Full text @ Nat. Methods
Citation
Yang, B., Lange, M., Millett-Sikking, A., Zhao, X., Bragantini, J., VijayKumar, S., Kamb, M., Gómez-Sjöberg, R., Solak, A.C., Wang, W., Kobayashi, H., McCarroll, M.N., Whitehead, L.W., Fiolka, R.P., Kornberg, T.B., York, A.G., Royer, L.A. (2022) DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy. Nature Methods. 19(4):461-469.
Abstract
The promise of single-objective light-sheet microscopy is to combine the convenience of standard single-objective microscopes with the speed, coverage, resolution and gentleness of light-sheet microscopes. We present DaXi, a single-objective light-sheet microscope design based on oblique plane illumination that achieves: (1) a wider field of view and high-resolution imaging via a custom remote focusing objective; (2) fast volumetric imaging over larger volumes without compromising image quality or necessitating tiled acquisition; (3) fuller image coverage for large samples via multi-view imaging and (4) higher throughput multi-well imaging via remote coverslip placement. Our instrument achieves a resolution of 450 nm laterally and 2 μm axially over an imaging volume of 3,000 × 800 × 300 μm. We demonstrate the speed, field of view, resolution and versatility of our instrument by imaging various systems, including Drosophila egg chamber development, zebrafish whole-brain activity and zebrafish embryonic development - up to nine embryos at a time.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping