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Fig. 2

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ZDB-FIG-220501-2

Publication

Yang et al., 2022 - DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy

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Fig. 2

Characterization of the microscope.

a, Imaging volume geometry. The coverslip is parallel to the xy plane. The optical axis of the microscope is along the z axis (depth). The sample is illuminated by an oblique light sheet in the x’y plane, where x’ is the light-sheet propagation direction. The field of view in the x’y plane is 800 μm (y) by 420 μm (x’), corresponding to 800 μm (y) by 300 μm (depth, z) in the yz plane. Volumetric data were acquired by scanning the sample, along the x axis, with respect to the illumination plane. By using light-sheet stabilized stage scanning, the scanning range (up to 75 mm, compared to 300 μm with galvo scanning) is only limited by the stage. b, Representative PSF obtained by imaging 100-nm green fluorescence beads. Projections along xy, xz and zy are shown. The PSF is slightly tilted and its long axis (z”) is about 20° with respect to the z axis. Taking this into consideration, the line profiles of the PSF were plotted and fitted along the three principal axes, that is x”, y and z”. The FWHM are, respectively, 479.9 ± 28.0, 379.2 ± 20.9 and 1,864.9 ± 174.3 nm (mean ± s.d., n = 156 fluorescence beads). Scale bar, 1 μm.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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