ZFIN ID: ZDB-FIG-220416-65
El Fersioui et al., 2022 - Premature Vertebral Mineralization in hmx1-Mutant Zebrafish. Cells   11(7) Full text @ Cells
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Figure 4 In situ hybridization detected bmp2b and bmp4 the dorsal region in Hmx1mut10 embryos: In situ hybridization in wildtype and hmx1mut10 embryos at 2 dpf. Staining for bmp2b, bmp4, and runx2b. No signal was detected for bmp2b and bmp4 in the dorsal region of wildtype zebrafish, while bmp4 was detected in the heart region ((A,C) red arrows). In hmx1mut10, bmp2b and bmp4 are expressed in the dorsal region (B,D). Runx2b in wildtype embryos at 2 dpf is expressed in the bone-forming regions (E). In hmx1mut10 embryos, runx2b is expressed in the dorsal region, and presents a similar pattern to bmp2b and bmp4 (F). Cl, cleithrum; ch, ceratohyal; cb, ceratobranchial; h, heart; op, opercle. Bar, A–F 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp2b WT standard conditions Long-pec Not Detected hindbrain ISH
hmx1iro2/iro2 standard conditions Long-pec hindbrain ISH
bmp4 WT standard conditions Long-pec heart ISH
Long-pec Not Detected hindbrain ISH
hmx1iro2/iro2 standard conditions Long-pec hindbrain ISH
runx2b WT standard conditions Long-pec ceratobranchial cartilage ISH
Long-pec ceratohyal cartilage ISH
Long-pec cleithrum ISH
Long-pec Not Detected hindbrain ISH
Long-pec opercle ISH
hmx1iro2/iro2 standard conditions Long-pec hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hmx1iro2/iro2 standard conditions Long-pec hindbrain bmp2b expression increased amount, abnormal
Long-pec hindbrain bmp4 expression increased amount, abnormal
Long-pec hindbrain runx2b expression increased amount, abnormal
Acknowledgments:
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