FIGURE

Figure 7

ID

ZDB-FIG-220411-7

Publication

Sheng et al., 2022 - MicroRNA-22 coordinates vascular and motor neuronal pathfinding via sema4 during zebrafish development

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Figure 7

Endothelial miR-c22a regulates PMNs axonal navigation. (a) A possible working model for how blood vessels regulate primary motor neuronal pathfinding in zebrafish. (b) Q-PCR analysis of the miR-22 expression in the isolated exosome from HUVECs (n = 6). (c) Imaging analysis of Tg(mnx1:EGFP::fli1a:CD61-mCherry); arrowhead indicates the vesicle from endothelial cells. (d) Confocal imaging analysis of control, rab11bb MO injected and GW4869 treated Tg(mnx1:EGFP) embryos. (e) Percentage of embryos with indicated phenotypes in control (n = 30), rab11bb MO injected (n = 55) and GW4869 treated Tg(mnx1:EGFP) embryos (n = 38). (f) Percentage of embryos with indicated phenotypes in control (n = 18), miR-22 MO injected (n = 25), miR-22 MO co-injected with exosome isolated from HUVECs (n = 28), miR-22 MO co-injected with exosome isolated from HUVECs transfected with miR-22 duplex (n = 32) and miR-22 MO co-injected with exosome isolated from HUVECs transfected with miR-22 MO Tg(mnx1:EGFP) embryos (n = 23). Fisher's exact test, ****p < 0.0001; ***p < 0.001; ns, no significance.

Expression Data

Genes:	GFP mCherry
Fish:	ml2Tg ml2Tg + MO1-rab11bb ml2Tg; zf3795Tg
Condition:	chemical treatment by environment: enzyme inhibitor
Knockdown Reagent:	MO1-rab11bb
Anatomical Terms:	CaP motoneuron axon primary motor neuron
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ml2Tg ml2Tg + MO1-rab11bb
Condition:	chemical treatment by environment: enzyme inhibitor
Knockdown Reagent:	MO1-rab11bb
Observed In:	CaP motoneuron axon extension involved in axon guidance
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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