FIGURE

Figure 11

ID

ZDB-FIG-220403-45

Publication

Laghi et al., 2022 - Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder

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Figure 11

SARS-CoV-2 does not replicate on EPC cells transfected with human ACE2. (A, B) Confocal microscopic images of EPC cells cultures 3 days after transfection. (A) Assessment of transfection efficiency at low magnification. (B) Demonstration of hACE2 expression at the membrane of cells transfected with both plasmids, high magnification, merge images of GFP, hACE2, and nuclei labels. (C, D) qRT-PCR measurement of N copies in cells. (C) Measurement at 2 days postexposure with virulent SARS-CoV-2, comparison of GFP and GFP+hACE2 overexpression. (D) Decline of RNA levels from day 0 to day 2 postexposure, and comparison of heat-inactivated and virulent virus.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Infect Microbiol