FIGURE

Figure 5

ID

ZDB-FIG-220403-40

Publication

Laghi et al., 2022 - Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder

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Figure 5

Immunodetection of infected cells in the swim bladder. (A) Scheme of the imaged region: the swim bladder is shown in yellow, the brain and spinal cord in magenta, the liver in gray. (B–F) Confocal images of SARS-CoV-2-injected (B, C, E–I) or uninjected (D) larvae fixed at 2 dpi and subjected to whole-mount immunohistochemistry with an anti-CoV-2-N antibody (green), an anti-GFAP antibody (red in B–D) or membranes stained by DiI (red in E–I), and stained nuclei (blue). (B–D) Maximal projections. The approximate contours of the partially collapsed swim bladders are shown with a dotted yellow line. N-positive cells shown with green arrowheads. Yellow arrows point to non-specific punctate signal in the notochord. (E–I) Single confocal planes.

Expression Data

Antibodies:	Ab1-SARS-CoV-2-nucleoprotein Ab22-gfap
Fish:	AB
Anatomical Terms:	spinal cord glial cell swim bladder
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Infect Microbiol