FIGURE

Figure 3.

ID

ZDB-FIG-220326-14

Publication

Vasileva et al., 2022 - Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth

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Figure 3.

Validation of <italic toggle='yes'>EWSR1-FLI</italic> expression in zebrafish embryos.

(A) Rate of embryos expressing EWSR1-FLI1 during the first 10 days of development (N = 524 Survival). Uninjected embryos (N = 528) as well as embryos injected with ubi:RSG-EWSR1-FLI1 plus GFP mRNA (N = 328) or ubi:RSG plus Cre mRNA (N = 304) were used as negative controls (p < 0.001 by log-rank test). (B) Timeline of zebrafish development after injection with ubi:RSG-EWSR1-FLI1 plus Cre mRNA (top panel). Zebrafish injected with ubi:RSG-EWSR1-FLI1 plus GFP mRNA (middle panel) or ubi:RSG plus Cre mRNA (bottom panel) were used as negative controls. Images were taken at 12 hpf, 24 hpf, and 5 dpf time points. (C) Representative image of embryos with low (GFP+), medium (GFP++), and high (GFP+++) levels of EWSR1-FLI1 expression. (D) Relative mRNA expression of EWSR1-FLI1 in embryos with low (GFP+), medium (GFP++), and high (GFP+++) levels of EWSR1-FLI1 according to eGFP signal. (E) Immunoblot confirming the expression of EWSR1-FLI1 protein in embryos with low (GFP+), medium (GFP++), and high (GFP+++) levels of EWSR1-FLI1 expression according to eGFP signal. (F) RNA scope staining of control and EWSR1-FLI1-positive embryos for nkx2.2a (529751-C2 RNAscope Probe – Dr-nkx2.2a-C2) and eGFP (538851 RNAscope Probe – EGFP-O4).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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