Figure 2
- ID
- ZDB-FIG-220326-12
- Publication
- Vasileva et al., 2022 - Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth
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- Figure 4—figure supplement 1.
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(A) Representative image of Ewing sarcoma tumor in WT zebrafish. H&E staining, immunohistochemistry (IHC) staining with anti-FLI1 and anti-CD99 antibodies. Scale bars, 100 μm. (B) H&E and Periodic acid-Schiff (PAS) staining of zebrafish tumors. Scale bars, 50 μm. (C) Relative mRNA expression of human EWSR1-FLI and zebrafish nr0b1 in normal and tumor tissues. Error bars represent standard error of the mean (SEM), N = 3, *p < 0.05, **p < 0.01, two-tailed Student’s t-test. (D) Immunofluorescence staining of zebrafish tumor with anti-eGFP and anti-FLI1 antibodies. Scale bars, 20 μm. (E) Validation of EWSR1-FLI1 expression in tumors at different sites by immunoblotting. (F) Gene set enrichment analysis (GSEA) comparing the enrichment of common upregulated proteins at dorsal, caudal and pectoral fin tumors to human Ewing sarcoma (GSE17674) and human Rhabdomyosarcoma (GSE108022) datasets.
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