FIGURE

FIGURE 7

ID

ZDB-FIG-220302-92

Publication

Babakhanova et al., 2021 - Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells

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FIGURE 7

Fluorescence imaging of TagRFP658, miRFP2, and emiRFP2 fusions expressed in HeLa cells. (a?e) Representative fluorescence images of live HeLa cells transfected with (a) TagRFP658-?-Tubulin (n = 11 cells from two independent transfections) and (b) TagRFP658-?-actin (n = 19 cells from two independent transfections). (c) miRFP2-?-Tubulin (n = 15 cells from two independent transfections), (d) miRFP2-?-actin (n = 21 cells from two independent transfections), and (e) Keratin-emiRFP2 (n = 8 cells from two independent transfections). Imaging conditions: (a?d) excitation 631/28 nm from an LED, emission 664LP under wide-field microscope. (e) Excitation 640 nm from laser, emission 670?750 nm under confocal microscope. (f) Dual-color near-infrared (NIR) imaging of live HeLa cells co-expressing Mito-mCardinal and H2B-emiRFP2 (n = 12 cells from two independent transfection)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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