FIGURE

Figure 6

ID

ZDB-FIG-220302-204

Publication

Confino et al., 2022 - A Zebrafish Model for a Rare Genetic Disease Reveals a Conserved Role for FBXL3 in the Circadian Clock System

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Figure 6

Clock-controlled aanat2 expression in the zebrafish pineal gland is affected by FBXL3a. Fbxl3a mutant larvae exhibit abnormal pineal aanat2 mRNA expression under constant dark (DD) and LD photoperiodic regimes (*** p < 0.001, t-test). Each value represents the mean optical density of pineal aanat2 expression using whole mount in situ hybridization (ISH) quantification (n = 15–20). ZT (hours) is indicated for each sample; white and black bars represent light and dark, respectively. Pineal aanat2 ISH quantification of 72–96 h post-fertilization (hpf) fbxl3a^−/− larvae and their WT siblings under LD (A) or DD (B) conditions, after entrainment by three LD cycles. (C) Representative pineal aanat2 ISH signal of 72–96 hpf fbxl3a^−/− larvae (bottom panel) and their WT siblings (top panel) under DD conditions. (D) Pineal aanat2 signal of a 96 hpf fbxl3a^−/− larva and its WT sibling after four LD cycles (at ZT2).

Expression Data

Gene:	aanat2
Fish:	WT fbxl3a^tlv08/tlv08
Conditions:	constant light altered light dark cycle
Anatomical Term:	epiphysis
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbxl3a^tlv08/tlv08
Conditions:	constant light altered light dark cycle
Observed In:	epiphysis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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