Figure 9

Behavioral antiseizure analysis of colchicine and indibulin in the zebrafish EKP seizure model after short- and long-term treatment. (A) Chemical structures of colchicine and indibulin. (B–I) Antiseizure activity of colchicine (B–E) and indibulin (F–I) after a treatment of 2 h (B,C,F,G) and after a treatment of 18 h (D,E,H,I) in the zebrafish ethyl ketopentenoate (EKP) seizure model. Behavioral data is expressed in mean actinteg units per 5 min (±SEM) during the 10–25 min recording period (B,D,F,H), and over consecutive 5 min time intervals during the entire 30 min recording period (C,E,G,I). Data are normalized against the vehicle (VHC) + EKP control condition (C,E,G,I). Data were pooled from three independent experiments with 10 replicate wells per test condition (n = 30) (B–I). Statistical analysis: one-way ANOVA with Dunnett’s multiple comparison test (B,D,F,H), two-way ANOVA with Dunnett’s multiple comparison test (C,E,G,I) (GraphPad Prism 9, San Diego, CA, USA). Significance levels: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.