Figure 7

Behavioral antiseizure analysis of plinabulin after short- and long-term treatment in the zebrafish EKP seizure model. Antiseizure activity of plinabulin in the zebrafish ethyl ketopentenoate (EKP) seizure model after a treatment of 2 h (A,B) and after a treatment of 18 h (C,D). Behavioral data is expressed in mean actinteg units per 5 min intervals (±SEM) during the 10–25 min recording period (A,C) and over consecutive 5 min time intervals during the entire 30 min recording period (B,D). Data is normalized against the vehicle (VHC) + EKP control condition (B,D). Data were pooled from four independent experiments with 10 replicate wells per test condition (n = 40) (A,B) and from three or four independent experiments with 10 replicate wells per test condition (n = 70 for VHC + VHC and VHC + EKP, n = 28–30 for 5–10 µM plinabulin conditions, and n = 39–40 for 0.156–2.5 µM plinabulin conditions) (C,D). Statistical analysis: one-way ANOVA with Dunnett’s multiple comparison test (A,C), two-way ANOVA with Dunnett’s multiple comparison test (B,D) (GraphPad Prism 9, San Diego, CA, USA). Significance levels: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.