Figure 5

Behavioral antiseizure analysis of plinabulin after a short- and long-term treatment in the zebrafish PTZ seizure model. Antiseizure activity of plinabulin in the zebrafish pentylenetetrazole (PTZ) seizure model after a treatment period of 2 h (A,B) and 18 h (C,D). Behavioral data is expressed in mean actinteg units per 5 min (±SEM) during the 30 min recording period (A,C) and over consecutive 5 min time intervals (B,D). Data is normalized against the vehicle (VHC) + PTZ control condition (B,D). Data were pooled from ten independent experiments with 10 replicate wells per test condition (n = 100 for VHC + VHC and VHC + PTZ and n = 70 for plinabulin + VHC and plinabulin + PTZ) (A,B), and from three independent experiments with 10 replicate wells per test condition (n = 30) (C,D). Statistical analysis: one-way ANOVA with Dunnett’s multiple comparison test (A,C), two-way ANOVA with Dunnett’s multiple comparison test (B,D) (GraphPad Prism 9, San Diego, CA, USA). Significance levels: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.