Fig. 6

Abnormal BB behaviors in vangl2m209 mutant FP. (A) BB movements in wt (left) and vangl2m209 (right) embryos during early (top) and late (bottom) developmental stages. Each dot represents the endpoint of a single BB movement, the starting point being the center of the circle; thus, the angles outside the circle represent BB movement orientation relative to the embryo AP axis, and the distance relative to the center of the circle represents its length (circle radius is 7 µm). The color of the dots indicates whether a movement leads to a membrane contact and, if so, the nature of the contact (anterior, posterior or lateral) (early stages: wild type: eight embryos, 20 cells, 238 movements; vangl2m209: nine embryos, 20 cells, 220 movements; late stages: wild type: four embryos, 19 cells, 255 movements; vangl2m209: four embryos, 42 cells, 708 movements). (B,C) Orientation of BB movements in wt and vangl2m209 embryos during early (B) or late (C) developmental stages. ns, P>0.05; *P<0.05 (Fisher's exact test). (D,E) Speed of BB movement, nature of membrane contacts and total time spent in contact with membranes during early (D) and late (E) developmental stages. Box boundaries represent the first and third quartiles of the distribution, and boxplot whiskers span 1.5 times the interquartile range of the distribution; each dot represents one cell. ****P<0.0001 (Wilcoxon rank sum test for BB speed and Fisher's exact test for BB/membrane contacts).