FIGURE

Fig 5

ID

ZDB-FIG-220214-23

Publication

Hamilton et al., 2022 - Enteric nervous system modulation of luminal pH modifies the microbial environment to promote intestinal health

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Fig 5

Decreased pH is necessary and sufficient for intestinal hyperinflammation.

(A) Quantification of luminal pH in intestinal bulb plotting red + green–blue integer values (see Methods) of CV WT and sox10 mutants after 24 hour exposure to 200 μM proton pump inhibitor omeprazole (Omz) or carbonic anhydrase inhibitor acetazolamide (ACTZ) 20 min after m-Cresol Purple gavage. (B) Quantification of intestinal neutrophil number per distal intestine in CV WT and sox10 mutants after 24 hour exposure to 200 μM Omz or ACTZ. (C) Representative images of WT after ACTZ treatment and (D) sox10 mutant after Omz treatment. In A & B, each dot is a fish, n>10. Boxes represent the first to third quartiles, center bar denotes the median, and whiskers the maximum and minimum of each dataset. * p < 0.05, **p<0.01, ****p<00001. ANOVA followed by Tukey’s post hoc test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox10^t3/t3 sox10^t3/+
Conditions:	chemical treatment by environment: acetazolamide chemical treatment by environment: omeprazole
Observed In:	gut neutrophil intestinal bulb pH elevation intestinal bulb pH reduction intestinal bulb regulation of pH
Stage:	Day 6

Phenotype Detail

Acknowledgments

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