FIGURE

Fig 3

ID

ZDB-FIG-220214-21

Publication

Hamilton et al., 2022 - Enteric nervous system modulation of luminal pH modifies the microbial environment to promote intestinal health

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Fig 3

sox10 mutants have altered intestinal tight junctions.

(A) Quantification of percent ZO1 colocalized with Occludin in the “ileum-like” region of CV and GF larvae. (B) Representative immunohistochemistry of CV WT and sox10 mutant ZO1, Occludin and colocalization in “ileum-like” region. (C) Quantification of percent ZO1 colocalized with Occludin in the “colon-like” region of CV and GF larvae. (D) Representative immunohistochemistry of ZO1, Occludin and colocalization in “colon-like” region. (E) Quantification of percent ZO1 colocalized with Claudin-15 in the ‘colon-like” region of CV and GF larvae. (F) Representative immunohistochemistry of ZO1, Claudin-15 and colocalization in “colon-like” region. Scale bar = 20μm. Each point in A, C and E is the average value of 2–3 sections per fish. Staining was performed on larvae from two independent GF derivations (n>7). Boxes represent the first to third quartiles, center bar denotes the median, and whiskers the maximum and minimum of each dataset. * p < 0.05. ANOVA followed by Tukey’s post hoc test.

Expression Data

Antibodies:	Ab1-cldn10 Ab1-tjp1 Ab2-ocln
Fish:	sox10^t3/t3 sox10^t3/+
Condition:	germ free
Anatomical Terms:	gut anterior side vent tight junction
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox10^t3/t3
Condition:	germ free
Observed In:	gut anterior side vent tight junction
Stage:	Day 6

Phenotype Detail

Acknowledgments

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