Figure 2

OP-B also had a significant inhibitory effect on A549/DDP carcinoma in situ in mice and transplanted tumors in zebrafish. A. Bioluminescent imaging and quantification of photon flux of 3 mg/kg OP-B treated groups with right lung parenchyma injection of luciferase-marked A549 and A549/DDP cells. B. Before and after OP-B treatment, the fluorescence intensity changes on model and OP-B groups of A549 and A549/DDP. C. Tumor growth rate of A549 (a) and A549/DDP (b) calculated according to the fluorescence intensity data. D. Comparison of tumor growth rates between A549 and A549/DDP. E. Representative images taken by fluorescence stereomicroscope of the zebra fish that were injected with A549 and A549/DDP cells and treated with 5 μM OP-B for 3 days. F. Relative fluorescence intensity of A549 (a) and A549/DDP (b) tumor in zebra fish after OP-B treatment. G. Comparison of tumor growth rate between A549 and A549/DDP that calculated according to the fluorescence intensity data. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001