FIGURE 5
Early light (EL) and early dark (ED) gene expression of arylalkylamine N-acetyltransferase (aanat2) and melatonin receptors (mtnr1aa and mtnr1bb) in adult Danio rerio. (A) Brain aanat2; (B) Brain mtnr1aa; (C) brain mtnr1bb. The gene expression was normalized by the 18S rRNA and expressed relative to the lowest mean of the control temperature group. The values are expressed as mean ± SEM. n = 3?6 (pools of four animals each). Significant differences are shown when p < 0.05. Red symbol corresponds to 28oC and blue symbol corresponds to 23oC. |