FIGURE

FIGURE 1

ID

ZDB-FIG-211216-173

Publication

Sua-Cespedes et al., 2021 - Low Temperature Effect on the Endocrine and Circadian Systems of Adult Danio rerio

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 1

Experimental design. (A) Danio rerio male adults (n = 144) were divided in two large groups: a control temperature group (animals maintained at 28^oC during 5 days and challenged 6 days at the same temperature) and a lower temperature group (animals maintained at 28^oC during 5 days and challenged 6 days at lower temperature 23^oC). After stimulation the animals were euthanized at early light (EL, 10 AM) and early dark (ED, 12 AM). (B) For the analysis, fish were separated into another two groups: a qPCR (96 animals) and a cortisol (48 animals) group. For the qPCR we evaluated gh1, aanat2, mtnr1aa, mtnr1bb, opn4m1, opn4m2 in the brain, ghra, ghrb, igf1a, igf1b, igf1ra, igf1rb, gr in the liver and muscle. Also, in all tissues analyzed we performed a qPCR for clock genes (per and cry). Each experimental n comprised pooled tissues from four fish. The total number of samples used in each temperature and time point was n = 3–6.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.