FIGURE

Fig. 5

ID

ZDB-FIG-211103-88

Publication

Stewart et al., 2021 - longfin causes cis-ectopic expression of the kcnh2a ether-a-go-go K+ channel to autonomously prolong fin outgrowth

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Fig. 5

kcnh2a is expressed ectopically in loft2 intra-ray mesenchyme lineage cells during fin regeneration. (A,B) kcnh2a mRNA localization (in green) detected by RNAscope in longitudinal caudal fin sections from 4 days post-amputation (dpa) TL (loft2/t2, A) and AB (B) animals. The green arrow indicates proximal intra-ray cells expressing high levels of kcnh2a, which is also detected in medial mesenchyme (yellow arrow) and distal cells (magenta arrow) of TL fish. The dashed yellow line indicates the site of amputation. Hoechst stained nuclei are in gray. Scale bars: 50 µm. (C,D) Double kcnh2a RNAscope (green) and Msx immunostaining (magenta) of 4 dpa caudal fin sections from TL (C) and AB (D) fish. (E,F) Combination kcnh2a RNAscope (green) and mCherry immunostaining (magenta) of 4 dpa fin sections from (E) tph1b:mCherry; loft2/+ and (F) tph1b:mCherry; lof+/+ fish. For C-F, green arrows highlight Msx+ or tph1b:mCherry+ cells with overlapping kcnh2a mRNA in proximal regenerating lof tissue. White arrows indicate Msx+ or tph1b:mCherry+ nuclei in corresponding regions from control fins lacking kcnh2a expression. Fin rays are outlined with a dashed orange line. Hoechst-stained nuclei are in gray. Scale bars: 50 µm.

Expression Data

Gene:	kcnh2a
Fish:	lof^t2/t2
Condition:	amputation: caudal fin
Anatomical Term:	caudal fin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lof^t2/t2
Condition:	amputation: caudal fin
Observed In:	caudal fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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