Fig 1

A Structure of EPT. B Epetraborole kill kinetics of M. abscessus in vitro. Bacteria were incubated in 7H9 complete with drug-free (black circles), AMK 20X MIC₉₀ (blue squares, 80 ?g/mL), RIF 20X MIC₉₀ (red triangles, 60 ?g/mL), EPT 20X MIC₉₀ (yellow hexagons, 1.6 ?g/mL), EPT 40X MIC₉₀ (orange diamonds, 3.2 ?g/mL), or EPT 80X MIC₉₀ (brown inverted triangles, 6.4 ?g/mL). The dashed red line indicates 99.9% or 3 log₁₀ reduction in CFU/mL threshold of a bactericidal drug. Data shown is mean ± SD of three independent experiments. C MIC₉₀ from induced cultures relative to drug free conditions. Bacteria were grown to OD₆₀₀ 0.05 and induced with no drug (white), 0.016 ?g/mL CLR (yellow), 0.016 ?g/mL EPT (blue). After 24 hours (left) or 14 days (right) of induction, cultures were passaged for 6 days in drug free media (dotted bars). Data shown is mean ± SD from three independent experiments. Error bars for drug free data derived from MIC₉₀ / MIC₉₀ average from three or six replicates. *, p = 0.02; **, p = 0.0017; ****, p = 0.0005 by one-way ANOVA with Tukey?s multiple comparisons test.