Figure 3

Renal tubular injury leads to loss of renal function. (A) Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive apoptotic cells (red) were detected in the renal tubules of Tg(cdh17:Dendra2-NTR) zebrafish larvae treated with MTZ, but not in the control (n = 30). Scale bar: 50 μm. (B,C) Tg(cdh17:Dendra2-NTR) zebrafish larvae treated with MTZ or the control were injected with 2 nL 5% FITC-inulin, and FITC-inulin fluorescence intensity of the caudal artery was detected in three separate regions 15 min and 4 h after injection. White or red arrows respectively indicated the same measuring position of Con larvae or MTZ larvae. (D) Zebrafish larvae treated with MTZ or control zebrafish larvae (30 larvae/Sample) were collected at 1 dpi to 4 dpi for the detection of UA and UN. (E) Tg(cdh17:Dendra2-NTR) zebrafish larvae treated with MTZ and control zebrafish larvae were collected (n = 60, respectively), and their survival rate was calculated from 1 dpi to 6 dpi. The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, and **** p < 0.001 (ANOVA/Dunnett’s test).