stat3 knockout impairs normal mitochondrial transcription and cell proliferation in the intestine and brain of 6 dpf zebrafish larvae. (A,A′) Relative mRNA expression of pcna (A) and mt_nd2 (A′) transcripts assayed by qRT-PCR in homogenised stat3−/− and WT siblings at 6 dpf; zgapdh was used as internal control (P-values=0.0358; 0.0182). (B,B′) Phospho-Histone-H3 (pH3) immunostaining of WT (B) and stat3−/− (B′) siblings at 6 dpf; inset shows magnification of boxed area of intestine. Arrowheads indicate pH3-positive cells. (C,C′) Hematoxylin and Eosin (H&E) staining on WT (C) and stat3−/− (C′) mutant sections at 6 dpf shows the complete loss of folding in the mutant intestinal epithelium; inset shows magnification of boxed area of intestine. (D) IF with anti-PCNA antibody on 6 dpf stat3−/− mutants showing decrease of fluorescence in the CNS (BF, bright field; Di, diencephalon; Tel, telencephalon; TeO, tectum opticum). (E) Fluorescence quantification of Pcna protein on lateral sections of 6 dpf stat3−/− mutants and WT siblings (n=8). Data are mean±s.e.m. *P<0.05; ****P<0.0001 (unpaired two-tailed t-test on three independent biological samples, where n not specified). Scale bars: 200 μm.
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