Stat3 mRNA is co-expressed with proliferation and mtDNA transcription markers in the TeO of zebrafish embryos. (A) WISH on 48 hpf zebrafish WT embryos using pcna (dark green frame and outline), mt_nd2 (light green frame and outline) and stat3 (yellow frame and outline) antisense mRNA probes shows co-expression of the three transcripts in the PML region of the TeO. PML, peripheral midbrain layer; r, retina. (B) IF against Pcna (green) and FISH with pcna antisense mRNA probe (red). (C) IF against Pcna (green) and FISH with mt_nd2 antisense mRNA probe (red). (D) IF against Pcna (green) and FISH with stat3 antisense mRNA probe (red). Images are representative of the 90% of processed samples. Scale bars: 100 μm.
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