FIGURE

Fig. 3.

ID

ZDB-FIG-210930-22

Publication

Peron et al., 2021 - Y705 and S727 are required for mitochondrial import and transcriptional activities of STAT3 and regulate proliferation of embryonic and tissue stem cells

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Fig. 3.

Mutation of Stat3 DBD (DNA binding domain) does not affect its mitochondrial activities. (A) WISH with anti-mt_nd2 antisense probe in uninjected 48 hpf larvae and in 48 hpf larvae injected with MLS_mStat3_NES or MLS_mStat3_ΔDNAbd_NES mRNA. (B) qRT-PCR showing mt_nd2 gene expression after injection of MLS_mStat3_NES or MLS_mStat3_ΔDNAbd_NES mRNA in 48 hpf embryos; zgapdh was used as internal control. (C) IF on ESCs transiently transfected with MLS_mStat3_NES or MLS_mStat3_ΔDNAbd_NES and stained with anti-STAT3 (green), anti-ATAD3 (red) antibodies and DAPI (blue). Data are mean±s.e.m. *P<0.05; **P<0.01 (unpaired two-tailed t-test on three independent biological samples, where n not specified). Scale bars: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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