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Whole-mount images and examples of perilipin-labeled lipid droplets corresponding to the transgenic zebrafish lines noted in <xref rid='table1' ref-type='table'>Table 1</xref>.All fish are heterozygous for the noted transgene. Heat shock transgenic lines were incubated at 37°C for 45 min prior to feeding. For whole-mount images, larvae were fed for 2 hr with a high-fat meal and imaged 3–4.5 hr (PLIN3 lines) or 5–8 hr (PLIN2 lines) following the start of the feed. Where appropriate, images of whole fish are included as insets. Scale = 500 µm for main images and insets. In the right column, confocal micrographs are included to show the fluorescent perilipin proteins labeling BODIPY-C12-positive lipid droplets in the various transgenic lines following a high-fat meal. Unless noted, images are from the intestine. Scale = 10 µm for each image. Images are representative of at least 10 fish from three independent clutches.
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