PUBLICATION

Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

Authors
Wilson, M.H., Ekker, S.C., Farber, S.A.
ID
ZDB-PUB-210814-10
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Ekker, Stephen C., Farber, Steven
Keywords
cell biology, developmental biology, zebrafish
MeSH Terms
  • Adipocytes/metabolism*
  • Adipose Tissue/metabolism
  • Animals
  • Animals, Genetically Modified
  • Homeostasis
  • Lipid Droplets/metabolism*
  • Lipid Metabolism
  • Perilipin-2/genetics
  • Perilipin-2/metabolism*
  • Perilipin-3/genetics
  • Perilipin-3/metabolism*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism*
PubMed
34387191 Full text @ Elife
Abstract
Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real-time from the subcellular to the whole organism level. Fluorescently tagging the lipid-droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes