Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
- Wilson, M.H., Ekker, S.C., Farber, S.A.
- eLIFE 10: (Journal)
- Registered Authors
- Ekker, Stephen C., Farber, Steven
- cell biology, developmental biology, zebrafish
- MeSH Terms
- Adipose Tissue/metabolism
- Animals, Genetically Modified
- Lipid Droplets/metabolism*
- Lipid Metabolism
- Zebrafish Proteins/metabolism*
- 34387191 Full text @ Elife
Wilson, M.H., Ekker, S.C., Farber, S.A. (2021) Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish. eLIFE. 10:.
Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real-time from the subcellular to the whole organism level. Fluorescently tagging the lipid-droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes