PUBLICATION

Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

Authors
Wilson, M.H., Ekker, S.C., Farber, S.A.
ID
ZDB-PUB-210814-10
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Ekker, Stephen C., Farber, Steven
Keywords
cell biology, developmental biology, zebrafish
MeSH Terms
  • Adipocytes/metabolism*
  • Zebrafish Proteins/metabolism*
  • Animals, Genetically Modified
  • Zebrafish/metabolism
  • Lipid Metabolism
  • Lipid Droplets/metabolism*
  • Perilipin-3/genetics
  • Perilipin-3/metabolism*
  • Perilipin-2/genetics
  • Perilipin-2/metabolism*
  • Animals
  • Adipose Tissue/metabolism
  • Homeostasis
(all 13)
PubMed
34387191 Full text @ Elife
Abstract
Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real-time from the subcellular to the whole organism level. Fluorescently tagging the lipid-droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models.
Errata / Notes
Corrected by: ZDB-PUB-230810-61
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
AB
c935Tg/+
c936Tg/+
c937Tg/+
c938Tg/+
c939Tg/+
plin2c874Tg/+
plin2c874Tg
plin2c874Tg/+; plin3c875Tg/+
plin2c874Tg; plin3c875Tg
1 - 10 of 13
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Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
TagRFPEFGTagRFP
1 - 2 of 2
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Mapping
No data available
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Citation
Wilson, M.H., Ekker, S.C., Farber, S.A. (2021) Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish. eLIFE. 10.