FIGURE

Figure 1

ID

ZDB-FIG-210911-11

Publication

Ear et al., 2021 - A long isoform of GIV/Girdin contains a PDZ-binding module that regulates localization and G-protein binding

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Figure 1

The C terminus of GIV has an evolutionarily conserved functional PDZ-binding motif downstream of its G-protein binding and/or modulatory domains.A, schematic depicting the major modules and motifs within GIV and Daple across different species. B, whole-mount RNA in situ hybridization of the CCDC88 gene family in developing zebrafish embryos across multiple time points. Inset shows anterior or dorsal view of select embryos. Scale bar = 1 mm. C, GST-pull-down assays were carried out using purified rat Gαi3 (loaded with GDP or GDP-AlF₄⁻) and lysates of HEK293T cells exogenous expressing zebrafish GIV-CT or Daple-CT. Bound proteins were analyzed (right) and equal loading of lysates was confirmed (left) by immunoblotting (IB). Arrows point to EGFP-zGIV-CT or EGFP-Daple-CT. D, GST-pull-down assays were carried out using purified GST-tagged PDZ domains of ParD3 and Dvl and lysates of HEK293T cells exogenous expressing zebrafish GIV-CT or Daple-CT and bound proteins were visualized as in C. GEM, guanine nucleotide-exchange modulator; GIV-L, long isoform of GIV; HOOK, a highly conserved microtubule-binding N-terminal domain; PBM, PDZ-binding motif.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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