FIGURE

Figure 5

ID

ZDB-FIG-210821-14

Publication

Del Bene et al., 2007 - In vivo validation of a computationally predicted conserved Ath5 target gene set

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Figure 5

In Vivo Occupancy of Four New Target Promoters by Ath5

Antibody directed against Ath5 was used to immunoprecipitate crosslinked chromatin fragments prepared from chicken neuroretina and optic tectum. Occupation was measured by real time PCR with primers specific for the predicted target genes (Dlx2, HuC, NN-1, and Int-α6). In one case, NN-1, the two predicted binding sites within the promoter region are at a distance sufficient to discriminate between the different occupancies of each of them with the resolution of the ChIP technique (about 700 bp). Both sites are in vivo occupied at different levels. In the y-axis, the absolute ChIP efficiency is indicated. NR6, neuroretina at day 6 post fertilization; NR9, neuroretina at day 9 post fertilization; OT6, optic tectum at day 6 post fertilization; d, distal; and p, proximal.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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