Fig. 5

Fig. 5

A Schematic of the experimental procedure. Wild-type fish were irradiated at 24 Gy 2 days before transplantation. Whole kidney marrow cells were prepared from 1-year stage asxl1^+/+ and asxl1^−/− donors and were dilute to 10⁵/μL. Each recipient was injected with 3 μL donor kidney marrow cells. Transplanted fish were raised in sterile water. After 18 days, surviving fish were collected for analysis (dpt, days posttransplantation). B May–Grünwald–Giemsa staining of whole kidney marrow of successful transplanted fish (asxl1^+/+, n = 3; asxl1^−/−, n = 4; scale bar, 20 μm; black boxes show enlarged details of neutrophils and macrophages/monocytes; green arrows, neutrophils; red arrow, macrophages). C Proportion of hematopoietic cells in whole kidney marrow of transplanted fish (asxl1^+/+, n = 3; asxl1^−/−, n = 4; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, error bars, mean ± SD). Mye P myeloid progenitors, Mye mature myeloid cells, Ery erythrocytes, Lym lymphocytes, Neu neutrophils, Mac macrophages, Eos eosinophils (color figure online).