FIGURE

Fig. 4

ID

ZDB-FIG-210714-4

Publication

Cirksena et al., 2021 - The C-mannosylome of human induced pluripotent stem cells implies a role for ADAMTS16 C-mannosylation in eye development

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Fig. 4

Targeting of dpy19l3 in medaka caused coloboma.A, schemes of dpy19l1 and dpy19l3 genes indicating exons (green bars), sgRNA positions, stop codons (asterisks). B, micrographs of medaka WT as well as dpy19l1 and dpy19l3 deletion mutants stage 40 and stage 28 of embryonic development grown at 28 °C. The gross of dpy19l1 mutants show caudal bending and shortening of anterior–posterior axis. A proportion of dpy19l3 mutants display defects in closure of the optic fissure, called coloboma (arrows). C, frequency of normal and deformed morphology as well as coloboma formation in medaka upon microinjection of GFP mRNA (control) or GFP mRNA in combination with sgRNAs targeting dpy19l1 or dpy19l3, respectively. Bars indicating normal (gray) and deformed (blue) morphology refer to the left y-axis, bars for coloboma formation (red) refer to right y-axis. Results of individual injection experiments are represented by dots. sgRNA, single-guide RNA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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