Fig. 2

TSR-containing proteins are reduced in cell culture supernatants of DPY19L1 KO and DPY19L3 KO hiPSCs. A, volcano plots displaying ratios of protein levels (fold change) detected in cell culture supernatants for DPY19L1 KO versus WT (left) and DPY19L3 KO versus WT (right). Significantly affected proteins with consensus sites for C-mannosylation are highlighted by blue (DPY19L1 KO) and red (DPY19L3 KO) dots, respectively. Dots above the horizontal line represent statistically significantly (p < 0.05, Student's t test) altered protein levels. B, enrichment of protein domains according to the Pfam database within the 25 proteins having consensus sites for C-mannosylation and showing significantly altered secretion in DPY19L1 KO and/or DPY19L3 KO hiPSCs (green bar, Fisher exact test, p ≤ 0.05). C, relative protein levels of 11 thrombospondin type 1 domain–containing proteins identified in the secretome of hiPSCs (WT, DPY19L1 KO, and DPY19L3 KO). Dots represent fold changes of protein levels in individual KO samples compared with the mean WT protein levels. The mean of individual fold changes for each KO is depicted by bars (blue bars for DPY19L1KO and red bars for DPY19L3 KO). Statistically significant fold changes are indicated by red asterisks. hiPSCs, human induced pluripotent stem cells; TSR, thrombospondin type 1 repeat.