Figure 6

Correlation between 3D image and sections of mouse embryo on E10 in a frozen block. The mouse embryo on E10 was frozen and sliced at a thickness of 10 µm using CoMBI-S system. (a) 3D volume-rendered image using 520 block-face images shows the surface structures of the mouse embryo. (b, c) The reconstructed sagittal planes. (d–f) Three examples of block-face images are shown. Green lines indicated the positions of (b) and (c). (g–i) Sections were collected at the positions of (d–f). H&E-stained sections can be correlated with block-face images in (d–f), and the positions where the sections were obtained are indicated as planes in (a). 1: Mandibular arch, 2: Forelimb, 3: Hindlimb, 4: Telencephalon, 5: Right ventricle, 6: Umbilical cord, 7: Dorsal root ganglion, 8: Somite, 9: Left ventricle, 10: Nasal pit, 11: Eye, 12: Otic vesicle, 13: Dorsal mesentery of hindgut, 14: Abdominal wall (broken). Bars: 1 mm (a, c), 500 µm (d, g).