PUBLICATION
Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
- Authors
- Ishii, N., Tajika, Y., Murakami, T., Galipon, J., Shirahata, H., Mukai, R., Uehara, D., Kaneko, R., Yamazaki, Y., Yoshimoto, Y., Iwasaki, H.
- ID
- ZDB-PUB-210625-4
- Date
- 2021
- Source
- Scientific Reports 11: 13108 (Journal)
- Registered Authors
- Murakami, Tohru
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 34162961 Full text @ Sci. Rep.
Citation
Ishii, N., Tajika, Y., Murakami, T., Galipon, J., Shirahata, H., Mukai, R., Uehara, D., Kaneko, R., Yamazaki, Y., Yoshimoto, Y., Iwasaki, H. (2021) Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens. Scientific Reports. 11:13108.
Abstract
Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the morphological analyses of various biological specimens, and its use is expanding. However, the conventional CoMBI system utilizes a cryostat, which limits its compatibility to only frozen blocks and the resolution of the block-face image. We developed a new CoMBI system that can be applied to not only frozen blocks but also paraffin blocks, and it has an improved magnification for block-face imaging. The new system, called CoMBI-S, comprises sliding-type sectioning devices and imaging devices, and it conducts block slicing and block-face imaging automatically. Sections can also be collected and processed for microscopy as required. We also developed sample preparation methods for improving the qualities of the block-face images and 3D rendered volumes. We successfully obtained correlative 3D datasets and 2D microscopic images of zebrafish, mice, and fruit flies, which were paraffin-embedded or frozen. In addition, the 3D datasets at the highest magnification could depict a single neuron and bile canaliculus.
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