Influence of C80 and S212 lipidation on membrane localization in vivo. (A) Expression of Wnt3-EGFP on the cell membrane (left) and a representative autocorrelation function (ACF; dots) and fit (line) of a Wnt3-EGFP FCS measurement at a cell membrane (right). (B) Expression of Wnt3S212A-EGFP on the cell membrane (left) and a representative autocorrelation function (ACF; dots) and fit (line) of a Wnt3S212A-EGFP FCS measurement at a cell boundary (right). (C) Expression of Wnt3C80-EGFP on the cell membrane (left) and a representative autocorrelation function (ACF; dots) and fit (line) of a Wnt3C80A-EGFP FCS measurement at a cell membrane (right). (D) Expression of Wnt3S212AC80-EGFP on the cell membrane (left) (Image brightness was increased for clear visualization) and a representative autocorrelation function (ACF; dots) and fit (line) of a Wnt3S212AC80A-EGFP FCS measurement at a cell membrane (right). The FCS curves were fitted using 2D-2particle-1triplet model. BV, fourth brain ventricle; Ce, cerebellum; OT, optic tectum. Orientation: anterior to the left. Scale bar 10 μm.
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