Figure 3
- ID
- ZDB-FIG-210617-22
- Publication
- Ellman et al., 2021 - Apex Resection in Zebrafish (Danio rerio) as a Model of Heart Regeneration: A Video-Assisted Guide
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Evaluation of zebrafish heart regeneration in 2D-analysis using histology and immunofluorescence. Cryosections from either sham operated (A,A?,A??) or apex resected (B?F, B??F?, B???F??). Zebrafish hearts were stained with Masson?s Trichrome (A?F) or immunostained with anti-MYH1 against myosin heavy chain (MYH1, red), wheat germ agglutinin (WGA, green) and 4?,6?-diamidino-2-phenylindole (DAPI, blue) (A??F? and A???F??). Five timepoints (dpi: days post injury) after apex resection were investigated: 1 dpi (B,B?,B??), 4 dpi (C,C?,C??), 7 dpi (D,D?,D??), 14 dpi (E,E?,E??) and 60 dpi (F,F?,F??). * marks fibrin clot. Scalebars: A?F = 300 µm, A??F? = 500 µm and A???F?? = 100 µm. |