Figure 7.

Impact of autophagy on long-term downregulation of MAP1LC3 expression in vivo. (A) Illustration of the experimental setting performed in Zebrafish larvae. (B) Representative immunoblot for LC3-II expression in Zebrafish larvae exposed for 4 h to clonidine or DMSO (used as control), collected directly after exposure (Day 0) or left to recover for 3 d. (B) Quantification of LC3-II versus ACTB expression from Zebrafish larvae at day 0 and day 3. All values are means ± SEM (n = 4, with 30 fish per condition). (C) map1lc3a, map1lc3b, sqstm1 and dnmt3a mRNA expression measured by RT-qPCR in zebrafish after the recovery period (n = 3, with 20 fish per condition). (D) Re-analysis of Map1lc3b gene expression in different mouse tissues such as liver, lens and lung before and after placenta withdrawal from 4 independent genome-wide data sets, with 0 corresponding to time of birth. All values are a means of at least 3 independent experiments ± SEM and considered significant for *p < 0.05, **p < 0.01, n.s., not significant for the indicated comparison