FIGURE 6

IL6 induces RG proliferation without stab wound injury in adult optic tectum. (A,B) Representative images of proliferative RG (BLBP+ PCNA+) in the contralateral side with PBS or 100 ng/μL IL6 at 1 dpi. Allow heads indicate BLBP+ PCNA+ cells and arrows indicate BLBP- PCNA+ cells. Scale bar: 100 μm in (A–D) and (A’,B’). (C) Schematic diagram of cerebroventricular microinjection. A small hole was made in the center of the skull above the right hemisphere of the optic tectum using a 30G needle. The injected solution spread through the corticospinal fluid. (D) Quantification of proliferative RG (BLBP+ PCNA+) in the injected and contralateral hemisphere with PBS (n = 4) or 100 ng/μL IL6 (n = 3) at 1 dpi. (E) Quantification of BLBP- PCNA+ cells in the injected and contralateral hemisphere with PBS or IL6 at 1 dpi. Statistical analyses were performed using one-way ANOVA with Tukey’s post hoc test.