FIGURE 1

Induction of RG proliferation during the early stages after stab wound injury. (A–D) Representative images of proliferative RG (BLBP+ PCNA+) in intact fish (A) and injured fish during the early stages of regeneration, 6, 12, and 24 hpi (B–D). (A’–D’) Magnified images of the boxed area in each image. White arrowheads indicate BLBP+ PCNA+ cells and dashed lines (B–D) indicate area injured by needle insertion. Scale bar: 100 μm in (A–D), 50 μm in (A’–D’). (E) Quantification of proliferative RG in intact fish (n = 4) and on the uninjured and injured hemispheres of injured fish at 6, 12, and 24 hpi. Statistical analyses between uninjured and injured hemispheres at each time point were evaluated using paired Student’s t-tests. (F) Quantification of proliferative cells except RG (BLBP-PCNA+) in intact fish (n = 4) and the uninjured and injured hemispheres of injured fish at 6, 12, and 24 hpi. Statistical analyses between uninjured and injured hemispheres at each time point were evaluated using paired Student’s t-tests.