FIGURE 4

Activation of IL6/Stat3 signaling during the early stages of optic tectum regeneration. (A–F) Quantitative real-time (qRT-) PCR analyses of IL6 superfamily cytokines il6 (A), il11a (B), and il11b (C), and Jak/Stat signaling components stat3 (D), jak1 (E), and gfap (F). Each graph indicates relative gene expression in the injured tectum from 1 to 7 dpi compared to intact optic tectum (n = 4 per gene per time point). Statistical analyses between intact and injured hemispheres at each time point were evaluated using unpaired Student’s t-tests. (G) Schematic diagram of the cell sorting workflow using the RG-specific reporter line, Tg(gfap:GFP). (H) Representative images of FACS plots for GFP-positive cells from the optic tectum of wild-type (left) or Tg(gfap:GFP) (right) fish. (I) Quantification of stat3 expression in GFPhigh-positive cells from uninjured and injured optic tectum at 24 hpi using qPCR. The graph shows relative stat3 expression in GFP^high-positive cells from injured tectum at 24 hpi compared to uninjured tectum (n = 11). Statistical analyses between uninjured and injured hemispheres was evaluated using unpaired Student’s t-tests.