Fig. 3

(A-A?) Dazl is not detectable in PGCs marked with Vasa at 30?hpf. (A?,A?) Enlargement of the yellow dotted box in A with Dazl (A?, green) and Vasa (A?, magenta). (B-F?) Single confocal plane of representative 12-day dazl^ae57/+ and dazl^ae57/ae57 gonads (B-C?), and dazl^+/+, dazl^?7/+ or dazl^?7/?7 14-day gonads (D-F?) immunostained with Dazl (green), Vasa (magenta) and DAPI (gray) as nuclear marker. Yellow dotted lines delineate GCs and blue lines outline somatic cells. (B-C?) Localization of Dazl in Vasa+ GC of dazl^ae57/+ (B-B?, n=1) and dazl^ae57/ae57 (C-C?, n=1). There was less abundant Dazl in dazl^ae57/ae57 gonads. (D-D?) Localization of Dazl in Vasa+ GC of dazl^+/+ (n=1). Image is 63× without 2.5× zoom to show full gonad at resolution 512×512. (E-E?) Localization of Dazl in Vasa+ GC of dazl^?7/+ (n=1). (F-F?) No detecatable Dazl in Vasa+ GC of dazl^?7/?7 (n=2). Yellow dotted line delineates the gonad. (G-G?) Twenty-nine-day gonads labeled with Dazl (green) and Vasa (magenta). DAPI (gray) marks nuclei. Dazl protein was diffusely localized in the cytoplasm of the GCs.