Fig. 5

Single confocal plane of representative dazl^?7/+ or dazl^?7/?7 day 8 gonads and dazl^ae57/+, dazl^?7/+ or dazl^?7/ae57 at 10?days labeled with Vasa (green), LaminB1 (magenta; nuclear envelope,) and DAPI (gray; DNA). (A-A?) Vasa+ GC of dazl^?7/+ (n=2) at day 8, with a folded raisin-like nuclear envelope. (B-B?) Vasa+ GC of dazl^?7/?7 (n=3) at day 8, with an unfolded smooth nuclear envelope of Vasa+ GC of dazlae^57/+ (C-C?, n=2) at 10?days. (D-D?) The change in nuclear appearance corresponds to amplification during cyst formation and is intact in dazlae^57/?7. (E-E?) After the transition at 10?days, the nuclei of Vasa+ GC of dazl^?7/+ remain smooth (n=2). The postmitotic GC nuclei also have a smooth nuclear membrane and a large nucleolus. (F-F?) Vasa+ GC of dazlae^57/?7 (n=5) at 10?days. Mutant GCs are individuals with a folded nuclear membrane rather than a smooth morphology at 10?days. Yellow dotted line delineates the gonad. Blue arrows indicate each note for the corresponding panel.