FIGURE

Fig. 4

ID

ZDB-FIG-210504-19

Publication

Peng et al., 2020 - Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration

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Fig. 4

Wnt signaling inhibition associates with CM dedifferentiation during heart regeneration. (A‒H) Immunofluorescence analyses showing increased emCMHC stained with N2.261 antibody and pS675-β-catenin at apical myocardial cells of the wound at 7 dpa (A‒D), which was reduced by wnt8a activation in injured Tg(hsp70:wnt8a) hearts (E‒H). N2.261 is not detectable in uninjured hearts (A and E) and overlaps mostly with induced pS675-β-catenin (D). (D and H) Merged panels of B and C and F and G, respectively. (I‒L) ISH analyses displaying gata4 expression at injured myocardial cell edges in heat-shocked Ctrl hearts (J) at 7 dpa, which was reduced in Tg(hsp:wnt8a) hearts (K) and increased in Tg(hsp:dkk1b) hearts (L) but hardly detectable in uninjured hearts (I). Brackets indicate amputation planes. Scale bar, 100 µm. (M and N) Bar charts depicting pS675-β-catenin (M) and emCMHC (N) levels in Ctrl (normalized as 1), Tg(hsp:wnt8a), and Tg(hsp:dkk1b) hearts. Fluorescent intensities were measured at the injury border zone using Image J. Data are mean ± SEM from five hearts for each group. Student’s t-test, *P < 0.05, **P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.