Fig. 2

RNA-seq analysis of ik KO embryos reveals downregulation of genes involved in skeletal muscle differentiation. a Distribution of RNA-seq profiling annotated to the biological process categories in 3 dpf ik KO embryos relative to WT. b Linear filter model showing overall gene expression correlation between WT and ik KO embryos. The Spearman correlation coefficient (R = 0.95) is indicated. c Heat map and hierarchical clustering of 7 differentially expressed skeletal muscle differentiation-related genes whose log2 of mRNA ratio was at least 2-fold in ik KO embryos relative to WT. Upregulated signals relative to the mean are indicated in red. Downregulated signals are in green color (scale bar, log 2 of mRNA ratio). d Relative mRNA expression levels of mybpc2a, mybpc1, tnnt2e, smyd1a, acta1a, and tnni3k in 3 dpf embryos as measured by quantitative RT-PCR. The average of three independent experiments is shown with error bars; 18S rRNA was used as a normalization control. **p < 0.01, ***p < 0.001