Functional study of bdkrb2 genes in zebrafish. Overexpression of Ssc_10023113 and Ssc_10023117 affects the phenotype and vascular development of embryos. An amount of 1.5 ng/embryo of mRNA was injected into Transgenic stain (Fli1a: EGFP) embryos at 1–4 cell stages. All embryos are shown with anterior to the left. a Phenotype of embryos with overexpression of mRNA at 48 h post-fertilization (hpf). Scale bar = 200 μm. a control group, embryos without injection. f control group, embryos with injection of equal volume of sterilized DEPC water. B–e, experimental group, embryos with injection of Ssc_10023113 mRNA. b normal phenotype; c mild phenotype; d medium phenotype; d serious phenotype. g–j, experimental group, embryos with injection of Ssc_10023117 mRNA. g normal phenotype; h mild phenotype; i medium phenotype; j serious phenotype. b Deformation percentages of embryos in each category as shown in (a). Phenotypes were divided into four categories: normal, shown in blue; mild, shown in purple; medium, shown in yellow; serious, shown in pink. N is the number of total samples analyzed in each group. c Vascular abnormalities of embryos with overexpression of mRNA at about 55 hpf. Scale bar = 100 μm. a’, control group, embryos with injection of equal volume of sterilized DEPC water. b’-d’, experimental group, embryos with injection of Ssc_10023113 mRNA. e’–g’, experimental group, embryos with injection of Ssc_10023117 mRNA. Yellow arrowheads indicate different types of vessels, whereas the red asterisks point out the abnormalities in the experimental group. H heart, CCV common cardinal veins, Se intersegmental vessel
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