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Fig. 5

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ZDB-FIG-210216-54

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Fallah et al., 2020 - Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis

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Fig. 5

Fig. 5. (A–C) Ex vivo effects of 0, 10 and 100 nM zGnih alone or in combination with 5 IU/ml hCG and 100 ng/ml (Fsh) on zebrafish spermatogenesis (G0/G1) cells (A), G2/M cells (B), spermatids (SPD) and spermatozoa (SPZ) cell populations (C). Zebrafish testes were dissociated after 7 days culture and assayed using FACScan analysis by FCM. The percentage of diploid and haploid cells were determined by BD CellQuest Pro for 0, 10, 100 nM zGnih treatments (mean ± SEM; n = 5). Counts were normalized to 100%, and treatment groups were normalized against their basal controls. Results were analyzed by ANOVA followed by Tukey's multiple comparison tests. Values with dissimilar superscripts are significantly different (P ≤ 0.05).

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Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 520, Fallah, H.P., Rodrigues, M.S., Zanardini, M., Nóbrega, R.H., Habibi, H.R., Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis, 111087, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.