Fig. 4

Fig. 4. A and D show the effects of 5 IU/ml hCG and 100 ng/ml Fsh on spermatogenesis after 7-days of zebrafish testis tissue culture on spermatogonial proliferation. (A and C) Proportion of type A undifferentiated spermatogonia (A_und), type A differentiated (A_diff) and type B spermatogonia (SpgB) were determined in the section surface area within the spermatogenic cysts in the absence (dotted line) or presence (black bars) of (A) 5 IU/ml hCG and (C) 100 ng/ml Fsh. Results were analyzed by Student's T-test (n = 5) and presented as fold changes with respect to the control group (basal). Values are mean ± SEM, and sterisks represent significant difference from the basal shown as dotted line. B and D show BrdU-incorporation during the last 6 h of 7-days testis culture. BrdU positive germ cells: A_und, A_diff and SpgB were counted in the testes incubated in culture medium in the presence of (B) 5 IU/ml hCG and (D) 100 ng/ml of Fsh. (B–D) BrdU labeling index of A_und, A_diff, and SpgB. Bars show the mean ± SEM (n = 5). Each treatment was compared with its own (individual) control (contralateral testis). Asterisks over connected line denote statistical significance between the control and treatment group, using Student's T-test.