Zygotic piRNA pathway activity. (A) Sense/antisense bias of piRNAs at the indicated time points (P-values generated using the Wilcoxon test). (B) Ping-pong signature was assessed at the different time points by plotting how many pairs of piRNAs from opposite genomic strands (y axis) display the overlap of a certain number of bases at their 5′ ends (x axis). Z-scores were calculated to test significance. Data are mean±s.d. for three replicates. (C) Hierarchical clustering of TE using the number of piRNAs mapping to those elements at the different time points. (D) Enrichment analysis of TE in the piRNA target clusters in C.
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