FIGURE

Figure 1

ID

ZDB-FIG-210206-1

Publication

Noschka et al., 2021 - Unbiased Identification of Angiogenin as an Endogenous Antimicrobial Protein With Activity Against Virulent Mycobacterium tuberculosis

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Figure 1

Screening a hemofiltrate library for activity against extracellular Mycobacterium tuberculosis (Mtb). (A) Around 48 fractions (each 10 μl) of pool seven of the hemofiltrate library were incubated for 96 h with extracellular Mtb. ³H-Uracil was added for the final 24 h of incubation. The uptake of ³H-Uracil was measured by scintillation counting in a ß-counter. Antimicrobial activity was calculated by comparison to the untreated control. The bars present the average antimycobacterial activity (%) calculated from triplicates of a single experiment. (B) Subfractions 7–16/17 were analyzed via mass spectrometry. Spectrum analysis yielded three distinct peaks, identifying Angiogenin with a molecular mass of 14,137 Dalton. (C) Angiogenin sequence coverage (88%) by MS/MS analysis of the sample after carbamidomethylation and digestion with trypsin. Full sequence (Angiogenin precursor): signal peptide: 1–24 and Angiogenin 25–147.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Microbiol